primary rabbit polyclonal irak4 antibody Search Results


rabbit anti irak4  (Novus Biologicals)
90
Novus Biologicals rabbit anti irak4
Rabbit Anti Irak4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti irak4/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
rabbit anti irak4 - by Bioz Stars, 2026-02
90/100 stars
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phospho irak4  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho irak4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Phospho Irak4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho irak4/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
phospho irak4 - by Bioz Stars, 2026-02
96/100 stars
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anti irak4  (ProSci Incorporated)
86
ProSci Incorporated anti irak4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Anti Irak4, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti irak4/product/ProSci Incorporated
Average 86 stars, based on 1 article reviews
anti irak4 - by Bioz Stars, 2026-02
86/100 stars
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irak4  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology irak4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Irak4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak4/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
irak4 - by Bioz Stars, 2026-02
93/100 stars
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rabbit monoclonal anti phospho irak4  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit monoclonal anti phospho irak4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Rabbit Monoclonal Anti Phospho Irak4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti phospho irak4/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit monoclonal anti phospho irak4 - by Bioz Stars, 2026-02
95/100 stars
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irak4  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc irak4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Irak4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak4/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
irak4 - by Bioz Stars, 2026-02
97/100 stars
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rabbit antibodies against irak-4  (Tularik Inc)
90
Tularik Inc rabbit antibodies against irak-4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Rabbit Antibodies Against Irak 4, supplied by Tularik Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antibodies against irak-4/product/Tularik Inc
Average 90 stars, based on 1 article reviews
rabbit antibodies against irak-4 - by Bioz Stars, 2026-02
90/100 stars
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irak 4  (ProSci Incorporated)
91
ProSci Incorporated irak 4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Irak 4, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak 4/product/ProSci Incorporated
Average 91 stars, based on 1 article reviews
irak 4 - by Bioz Stars, 2026-02
91/100 stars
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interleukin 1 receptor associated kinase 4(irak4) rabbit polyclonal antibody antibody a6208  (ABclonal Biotechnology)
90
ABclonal Biotechnology interleukin 1 receptor associated kinase 4(irak4) rabbit polyclonal antibody antibody a6208
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Interleukin 1 Receptor Associated Kinase 4(Irak4) Rabbit Polyclonal Antibody Antibody A6208, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/interleukin 1 receptor associated kinase 4(irak4) rabbit polyclonal antibody antibody a6208/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
interleukin 1 receptor associated kinase 4(irak4) rabbit polyclonal antibody antibody a6208 - by Bioz Stars, 2026-02
90/100 stars
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anti irak4  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti irak4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Anti Irak4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti irak4/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
anti irak4 - by Bioz Stars, 2026-02
96/100 stars
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rabbit anti-human irak-4  (Biomol GmbH)
90
Biomol GmbH rabbit anti-human irak-4
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
Rabbit Anti Human Irak 4, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human irak-4/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
rabbit anti-human irak-4 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
1 ap  (Proteintech)
93
Proteintech 1 ap
Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of <t>IRAK4.</t> The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 ap/product/Proteintech
Average 93 stars, based on 1 article reviews
1 ap - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

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Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of IRAK4. The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.

Journal: The Journal of Biological Chemistry

Article Title: Toll-like Receptors as a Target of Food-derived Anti-inflammatory Compounds *

doi: 10.1074/jbc.M114.585901

Figure Lengend Snippet: Inhibition of TLR dimerization by iberin. A, effect of iberin on the binding of LPS to RAW264.7 cells. The cells were pretreated with iberin (0–20 μm) or polymyxin B (PB; 100 μg/ml) for 30 min and then treated with 300 ng/ml biotin-labeled LPS (Biotin-LPS) for 15 min. After incubation, the cells were analyzed by ELISA using HRP-conjugated avidin. Error bars represent S.D. B, the TLR/NF-κB signaling pathway. C, effect of iberin on NF-κB activation mediated by TLR2. The HEK293 cells were transiently co-transfected with the expression plasmid for TLR2 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. #, p < 0.05 versus control; *, p < 0.05 versus TLR2 overexpression (0 μm iberin). Error bars represent S.D. D, effect of iberin on NF-κB activation mediated by MyD88. HEK293 cells were transiently co-transfected with the expression plasmid for adaptor protein MyD88 or empty vector and NF-κB reporter plasmid. After transfection, the cells were cultured in the absence or presence of iberin for 24 h. Error bars represent S.D. E, effect of iberin on poly(I:C)-induced phosphorylation of TBK1 (p-TBK1). RAW264.7 cells were pretreated with iberin (10 μm) for 30 min and then treated with poly(I:C) (10 μg/ml) for 30 min. F, effect of iberin on co-immunoprecipitation of MyD88 with TLR4. The HEK293 cells were transfected with expression vectors encoding TLR4-His and/or MyD88 together with MD2. Twenty-four hours after transfection, the cells were pretreated with iberin (15 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. After incubation, the cells were washed and lysed. The cell lysates were immunoprecipitated (IP) with the anti-His-tag antibody, and the immunoprecipitates were analyzed by immunoblotting using the anti-MyD88 and anti-His tag antibodies. G, immunoblot analysis of phosphorylation of IRAK4. The RAW 264.7 cells were pretreated with iberin for 60 min and then treated with LPS (100 ng/ml) for 30 min. H, inhibition of TLR4 dimerization by iberin. The HEK293 cells expressing TLR4-HA and/or TLR4-His together with MD2 were pretreated with iberin (20 μm) for 30 min and then treated with LPS (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with anti-His tag antibody and immunoblotted with the anti-HA tag (upper) or anti-His tag (lower) antibody. I, inhibition of TLR2/TLR6 heterodimerization by iberin. The HEK293 cells expressing TLR2-His and/or TLR6-HA were pretreated with iberin (20 μm) for 30 min and then treated with Pam2CSK4 (500 ng/ml) for 20 min. The cells were then subjected to immunoprecipitation with the anti-His tag antibody and immunoblotted with anti-HA tag (upper) or anti-His tag (lower) antibody.

Article Snippet: The antibodies against ERK, phospho-ERK, JNK, phospho-JNK, p38, phospho-p38, interleukin 1 receptor-associated kinase 4 (IRAK4), phospho-IRAK4, phospho-TBK1, and TBK1 were obtained from Cell Signaling Technology.

Techniques: Inhibition, Binding Assay, Labeling, Incubation, Enzyme-linked Immunosorbent Assay, Avidin-Biotin Assay, Activation Assay, Transfection, Expressing, Plasmid Preparation, Cell Culture, Over Expression, Immunoprecipitation, Western Blot